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cd19 b cells  (Miltenyi Biotec)


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    Miltenyi Biotec cd19 b cells
    Cd19 B Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 8882 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd19+cells/LS+Columns/pm42272255-106-11-24
    Average 99 stars, based on 8882 article reviews
    cd19 b cells - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Deep Proteomics Analysis Unravels the Molecular Signatures of Tonsillar B Cells in PFAPA and OSAS in the Pediatric Population
    Article Snippet: Then, the freezing medium was discarded, and the cells were resuspended in cold PBS and counted by Denovix Celldrop BF (DeNovix Inc., Wilmington, DE, USA) with trypan blue. .. CD19+ cells were isolated using Miltenyi magnetic microbeads (130-050-301) using the LS columns (130-042-401) according to the manufacturer’s instructions. .. Upon being captured by the magnetic beads, the cells were centrifuged, and SDS lysis buffer (Thermo Scientific A40006) was added to the pellet, which was then heated at 95 °C for 5 min.

    Article Title: Selective expansion of T-cell receptor engineered T cells with increased stem-like phenotypes using neoantigen stimulation.
    Article Snippet: .. Primary autologous B cells were generated from CD19+ cells isolated from autologous apheresis by positive selection using CD19+ microbeads (Miltenyi Biotec, catalog no. 130- 050- 301) and were co- incubated with irradiated NIH3T3 cells constitutively expressing human CD40 ligand in a B- cell medium (Iscove’s Modified Dulbecco’s Medium (Thermo Fisher, catalog no. 12440053) supplemented with 10% human serum, 1% penicillinstreptomycin, and 1% GlutaMAX) in the presence of 200 U/mL IL- 4. ..

    Article Title: Deep Proteomics Analysis Unravels the Molecular Signatures of Tonsillar B Cells in PFAPA and OSAS in the Pediatric Population.
    Article Snippet: Then, the freezing medium was discarded, and the cells were resuspended in cold PBS and counted by Denovix Celldrop BF (DeNovix Inc., Wilmington, DE, USA) with trypan blue. .. CD19+ cells were isolated using Miltenyi magnetic microbeads (130-050-301) using the LS columns (130-042-401) according to the manufacturer’s instructions. .. Upon being captured by the magnetic beads, the cells were centrifuged, and SDS lysis buffer (Thermo Scientific A40006) was added to the pellet, which was then heated at 95 ◦C for 5 min.

    Article Title: Development of Monoclonal Antibodies for Identifying Plant-Parasitic Nematodes
    Article Snippet: .. CD19 + cells were isolated using Mouse CD19 magnetic MicroBeads and LS columns (Miltenyi Biotec, Auburn CA, USA) according to the manufacturer's protocol. .. Cells were resuspended at 1 × 10 6 concentration in 1 mL solution containing 100 μL BD Brilliant Staining Buffer Plus (BD Biosciences, Franklin Lakes NJ, USA) and a mixture of dye-labelled rat anti-mouse antibodies (CD20, CD3, CD19, BC45R/B220a, IGD and IGG) for cell sorting using flow cytometry.

    Generated:

    Article Title: Selective expansion of T-cell receptor engineered T cells with increased stem-like phenotypes using neoantigen stimulation.
    Article Snippet: .. Primary autologous B cells were generated from CD19+ cells isolated from autologous apheresis by positive selection using CD19+ microbeads (Miltenyi Biotec, catalog no. 130- 050- 301) and were co- incubated with irradiated NIH3T3 cells constitutively expressing human CD40 ligand in a B- cell medium (Iscove’s Modified Dulbecco’s Medium (Thermo Fisher, catalog no. 12440053) supplemented with 10% human serum, 1% penicillinstreptomycin, and 1% GlutaMAX) in the presence of 200 U/mL IL- 4. ..

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells
    Article Snippet: .. EBV transformed lymphoblastoid 438 cell lines (LCL) from allogeneic human fetal liver tissue were generated by isolating CD19+ cells by 439 magnetic activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with 440 Epstein-Barr Virus (EBV) B95-8; produced as previously described57 at a multiplicity of infection (MOI) 441 of 0.05-0.15. .. NALM6 was transduced with the pCDH-EF1-Luciferase-T2A-GFP lentiviral vector and 442 sorted using a BD FACSAria (Becton Dickinson).

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells
    Article Snippet: .. EBV-transformed LCLs from allogeneic human fetal liver tissue were generated by isolating CD19 + cells by magnetic-activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with EBV B95-8, produced as previously described at a multiplicity of infection (MOI) of 0.05–0.15. .. NALM6 was transduced with the pCDH-EF1α-Luciferase-T2A-GFP lentiviral vector and sorted using a BD FACSAria (Becton Dickinson).

    Selection:

    Article Title: Selective expansion of T-cell receptor engineered T cells with increased stem-like phenotypes using neoantigen stimulation.
    Article Snippet: .. Primary autologous B cells were generated from CD19+ cells isolated from autologous apheresis by positive selection using CD19+ microbeads (Miltenyi Biotec, catalog no. 130- 050- 301) and were co- incubated with irradiated NIH3T3 cells constitutively expressing human CD40 ligand in a B- cell medium (Iscove’s Modified Dulbecco’s Medium (Thermo Fisher, catalog no. 12440053) supplemented with 10% human serum, 1% penicillinstreptomycin, and 1% GlutaMAX) in the presence of 200 U/mL IL- 4. ..

    Incubation:

    Article Title: Selective expansion of T-cell receptor engineered T cells with increased stem-like phenotypes using neoantigen stimulation.
    Article Snippet: .. Primary autologous B cells were generated from CD19+ cells isolated from autologous apheresis by positive selection using CD19+ microbeads (Miltenyi Biotec, catalog no. 130- 050- 301) and were co- incubated with irradiated NIH3T3 cells constitutively expressing human CD40 ligand in a B- cell medium (Iscove’s Modified Dulbecco’s Medium (Thermo Fisher, catalog no. 12440053) supplemented with 10% human serum, 1% penicillinstreptomycin, and 1% GlutaMAX) in the presence of 200 U/mL IL- 4. ..

    Irradiation:

    Article Title: Selective expansion of T-cell receptor engineered T cells with increased stem-like phenotypes using neoantigen stimulation.
    Article Snippet: .. Primary autologous B cells were generated from CD19+ cells isolated from autologous apheresis by positive selection using CD19+ microbeads (Miltenyi Biotec, catalog no. 130- 050- 301) and were co- incubated with irradiated NIH3T3 cells constitutively expressing human CD40 ligand in a B- cell medium (Iscove’s Modified Dulbecco’s Medium (Thermo Fisher, catalog no. 12440053) supplemented with 10% human serum, 1% penicillinstreptomycin, and 1% GlutaMAX) in the presence of 200 U/mL IL- 4. ..

    Expressing:

    Article Title: Selective expansion of T-cell receptor engineered T cells with increased stem-like phenotypes using neoantigen stimulation.
    Article Snippet: .. Primary autologous B cells were generated from CD19+ cells isolated from autologous apheresis by positive selection using CD19+ microbeads (Miltenyi Biotec, catalog no. 130- 050- 301) and were co- incubated with irradiated NIH3T3 cells constitutively expressing human CD40 ligand in a B- cell medium (Iscove’s Modified Dulbecco’s Medium (Thermo Fisher, catalog no. 12440053) supplemented with 10% human serum, 1% penicillinstreptomycin, and 1% GlutaMAX) in the presence of 200 U/mL IL- 4. ..

    Modification:

    Article Title: Selective expansion of T-cell receptor engineered T cells with increased stem-like phenotypes using neoantigen stimulation.
    Article Snippet: .. Primary autologous B cells were generated from CD19+ cells isolated from autologous apheresis by positive selection using CD19+ microbeads (Miltenyi Biotec, catalog no. 130- 050- 301) and were co- incubated with irradiated NIH3T3 cells constitutively expressing human CD40 ligand in a B- cell medium (Iscove’s Modified Dulbecco’s Medium (Thermo Fisher, catalog no. 12440053) supplemented with 10% human serum, 1% penicillinstreptomycin, and 1% GlutaMAX) in the presence of 200 U/mL IL- 4. ..

    Transformation Assay:

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells
    Article Snippet: .. EBV transformed lymphoblastoid 438 cell lines (LCL) from allogeneic human fetal liver tissue were generated by isolating CD19+ cells by 439 magnetic activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with 440 Epstein-Barr Virus (EBV) B95-8; produced as previously described57 at a multiplicity of infection (MOI) 441 of 0.05-0.15. .. NALM6 was transduced with the pCDH-EF1-Luciferase-T2A-GFP lentiviral vector and 442 sorted using a BD FACSAria (Becton Dickinson).

    FACS:

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells
    Article Snippet: .. EBV transformed lymphoblastoid 438 cell lines (LCL) from allogeneic human fetal liver tissue were generated by isolating CD19+ cells by 439 magnetic activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with 440 Epstein-Barr Virus (EBV) B95-8; produced as previously described57 at a multiplicity of infection (MOI) 441 of 0.05-0.15. .. NALM6 was transduced with the pCDH-EF1-Luciferase-T2A-GFP lentiviral vector and 442 sorted using a BD FACSAria (Becton Dickinson).

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells
    Article Snippet: .. EBV-transformed LCLs from allogeneic human fetal liver tissue were generated by isolating CD19 + cells by magnetic-activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with EBV B95-8, produced as previously described at a multiplicity of infection (MOI) of 0.05–0.15. .. NALM6 was transduced with the pCDH-EF1α-Luciferase-T2A-GFP lentiviral vector and sorted using a BD FACSAria (Becton Dickinson).

    Infection:

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells
    Article Snippet: .. EBV transformed lymphoblastoid 438 cell lines (LCL) from allogeneic human fetal liver tissue were generated by isolating CD19+ cells by 439 magnetic activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with 440 Epstein-Barr Virus (EBV) B95-8; produced as previously described57 at a multiplicity of infection (MOI) 441 of 0.05-0.15. .. NALM6 was transduced with the pCDH-EF1-Luciferase-T2A-GFP lentiviral vector and 442 sorted using a BD FACSAria (Becton Dickinson).

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells
    Article Snippet: .. EBV-transformed LCLs from allogeneic human fetal liver tissue were generated by isolating CD19 + cells by magnetic-activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with EBV B95-8, produced as previously described at a multiplicity of infection (MOI) of 0.05–0.15. .. NALM6 was transduced with the pCDH-EF1α-Luciferase-T2A-GFP lentiviral vector and sorted using a BD FACSAria (Becton Dickinson).

    Virus:

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells
    Article Snippet: .. EBV transformed lymphoblastoid 438 cell lines (LCL) from allogeneic human fetal liver tissue were generated by isolating CD19+ cells by 439 magnetic activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with 440 Epstein-Barr Virus (EBV) B95-8; produced as previously described57 at a multiplicity of infection (MOI) 441 of 0.05-0.15. .. NALM6 was transduced with the pCDH-EF1-Luciferase-T2A-GFP lentiviral vector and 442 sorted using a BD FACSAria (Becton Dickinson).

    Produced:

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells
    Article Snippet: .. EBV transformed lymphoblastoid 438 cell lines (LCL) from allogeneic human fetal liver tissue were generated by isolating CD19+ cells by 439 magnetic activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with 440 Epstein-Barr Virus (EBV) B95-8; produced as previously described57 at a multiplicity of infection (MOI) 441 of 0.05-0.15. .. NALM6 was transduced with the pCDH-EF1-Luciferase-T2A-GFP lentiviral vector and 442 sorted using a BD FACSAria (Becton Dickinson).

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells
    Article Snippet: .. EBV-transformed LCLs from allogeneic human fetal liver tissue were generated by isolating CD19 + cells by magnetic-activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with EBV B95-8, produced as previously described at a multiplicity of infection (MOI) of 0.05–0.15. .. NALM6 was transduced with the pCDH-EF1α-Luciferase-T2A-GFP lentiviral vector and sorted using a BD FACSAria (Becton Dickinson).



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    cd4 cd8α cd19 cd56 cells - by Bioz Stars, 2026-09
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    The APC function of B cells is closely associated with their FA metabolism in metastatic OvCa. A GSVA pathway enrichment analysis of APC function low and APC function high B cells in patients with metastatic OvCa ( GSE235951 , GSE147082 and GSE154600 dataset in the GEO database, n = 13). B Representative images of the IHC staining of TLS structure (composed of CD3 + T cells, <t>CD19</t> + B cells, and CD21 + FDC), CD80, and CD86, respectively, in the area adjacent to or away from tumor or adipose tissues in clinical HGSOC specimens (n = 20 for TLS and n = 5 for CD80 or CD86). Red dashed line area: TLS structure. Magnification × 200. C Representative image of lymphoid aggregates (white dashed line area) by immunofluorescent staining in ascites of OvCa mice with 3 w and 6 w in tumor-bearing mice. D Mean fluorescence intensity of CD80, CD86, CD83, and MHC class II molecules in ascitic CD19 + B cells of OvCa mice were detected by flow cytometry. E Comparison of mRNA levels of FA metabolic genes in ascitic B cells in OvCa mice. The relative expression of each gene was calculated using β -actin as the internal reference. F Protein expression of β-actin and FA metabolic proteins in ascitic B cells in OvCa mice was assessed by WB. β -Actin was used as the internal control to calculate the relative expression level of FA metabolic proteins. G Mean fluorescence intensity of Bodipy C16 in ascitic B cells of OvCa mice detected by flow cytometry. H Expression of A-CoA in ascitic B cells of OvCa mice detected by ELISA. I Expression of ATP in ascitic B cells of OvCa mice detected by ELISA. J Mean fluorescence intensity of intracellular oxidized lipid in ascitic B cells of OvCa mice detected by flow cytometry. I Comparison of mRNA levels of FA metabolic genes in ascitic B cells in OvCa mice. The relative expression of each gene was calculated using β -actin as the internal reference. Data are presented as the mean ± SD of three independent experiments. FA, fatty acid; TLS, tertiary lymphoid structure. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, not significant
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    The APC function of B cells is closely associated with their FA metabolism in metastatic OvCa. A GSVA pathway enrichment analysis of APC function low and APC function high B cells in patients with metastatic OvCa ( GSE235951 , GSE147082 and GSE154600 dataset in the GEO database, n = 13). B Representative images of the IHC staining of TLS structure (composed of CD3 + T cells, CD19 + B cells, and CD21 + FDC), CD80, and CD86, respectively, in the area adjacent to or away from tumor or adipose tissues in clinical HGSOC specimens (n = 20 for TLS and n = 5 for CD80 or CD86). Red dashed line area: TLS structure. Magnification × 200. C Representative image of lymphoid aggregates (white dashed line area) by immunofluorescent staining in ascites of OvCa mice with 3 w and 6 w in tumor-bearing mice. D Mean fluorescence intensity of CD80, CD86, CD83, and MHC class II molecules in ascitic CD19 + B cells of OvCa mice were detected by flow cytometry. E Comparison of mRNA levels of FA metabolic genes in ascitic B cells in OvCa mice. The relative expression of each gene was calculated using β -actin as the internal reference. F Protein expression of β-actin and FA metabolic proteins in ascitic B cells in OvCa mice was assessed by WB. β -Actin was used as the internal control to calculate the relative expression level of FA metabolic proteins. G Mean fluorescence intensity of Bodipy C16 in ascitic B cells of OvCa mice detected by flow cytometry. H Expression of A-CoA in ascitic B cells of OvCa mice detected by ELISA. I Expression of ATP in ascitic B cells of OvCa mice detected by ELISA. J Mean fluorescence intensity of intracellular oxidized lipid in ascitic B cells of OvCa mice detected by flow cytometry. I Comparison of mRNA levels of FA metabolic genes in ascitic B cells in OvCa mice. The relative expression of each gene was calculated using β -actin as the internal reference. Data are presented as the mean ± SD of three independent experiments. FA, fatty acid; TLS, tertiary lymphoid structure. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, not significant

    Journal: Cancer Immunology, Immunotherapy : CII

    Article Title: Promoting APC function of B cells via reprogramming the fatty acid metabolism enhances anticancer immunity in metastatic ovarian cancer

    doi: 10.1007/s00262-026-04387-y

    Figure Lengend Snippet: The APC function of B cells is closely associated with their FA metabolism in metastatic OvCa. A GSVA pathway enrichment analysis of APC function low and APC function high B cells in patients with metastatic OvCa ( GSE235951 , GSE147082 and GSE154600 dataset in the GEO database, n = 13). B Representative images of the IHC staining of TLS structure (composed of CD3 + T cells, CD19 + B cells, and CD21 + FDC), CD80, and CD86, respectively, in the area adjacent to or away from tumor or adipose tissues in clinical HGSOC specimens (n = 20 for TLS and n = 5 for CD80 or CD86). Red dashed line area: TLS structure. Magnification × 200. C Representative image of lymphoid aggregates (white dashed line area) by immunofluorescent staining in ascites of OvCa mice with 3 w and 6 w in tumor-bearing mice. D Mean fluorescence intensity of CD80, CD86, CD83, and MHC class II molecules in ascitic CD19 + B cells of OvCa mice were detected by flow cytometry. E Comparison of mRNA levels of FA metabolic genes in ascitic B cells in OvCa mice. The relative expression of each gene was calculated using β -actin as the internal reference. F Protein expression of β-actin and FA metabolic proteins in ascitic B cells in OvCa mice was assessed by WB. β -Actin was used as the internal control to calculate the relative expression level of FA metabolic proteins. G Mean fluorescence intensity of Bodipy C16 in ascitic B cells of OvCa mice detected by flow cytometry. H Expression of A-CoA in ascitic B cells of OvCa mice detected by ELISA. I Expression of ATP in ascitic B cells of OvCa mice detected by ELISA. J Mean fluorescence intensity of intracellular oxidized lipid in ascitic B cells of OvCa mice detected by flow cytometry. I Comparison of mRNA levels of FA metabolic genes in ascitic B cells in OvCa mice. The relative expression of each gene was calculated using β -actin as the internal reference. Data are presented as the mean ± SD of three independent experiments. FA, fatty acid; TLS, tertiary lymphoid structure. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, not significant

    Article Snippet: In the mechanistic study, mouse ascitic CD19 + B cells (1 × 10 6 /ml) were pretreated with fatty acid binding protein 4 gene (FABP4) inhibitor (BMS309403, MedChemExpress; Cat# HY-101903; 50 μM), PPARγ antagonist (GW9662, MedChemExpress; Cat# HY-16578; 25 μM), and PPAR γ agonist (Troglitazone, Trog, MedChemExpress; Cat# HY-50935; 10 μM) for 2h, respectively.

    Techniques: Immunohistochemistry, Staining, Fluorescence, Flow Cytometry, Comparison, Expressing, Control, Enzyme-linked Immunosorbent Assay

    The APC function and resulting anticancer immunity of B cells can be enhanced by oleic acid (OA) via reprogramming FA metabolism in vitro. A Mean fluorescence intensity of CD80 and MHC Class II molecules in CD19 + B cells from peripheral blood of healthy volunteers (n = 3) treated with OA and PA (both 150 μM), respectively. B Mean fluorescence intensity of CD80, CD86, CD83, MHC Class II molecules, and Ki67 in splenic CD19 + B cells of WT mice treated with 150 μM OA. C Mean fluorescence intensity of CD80, CD86, CD83, and MHC Class II molecules in ascitic CD19 + B cells from 3 w OvCa-bearing mice when treated with 150 μM OA. D Analysis of FA metabolism-related signaling pathways based on RNA-seq results. GSEA was used to analysised the FA metabolic pathways. E Comparison of mRNA levels of main FA metabolic genes in ascitic B cells from 3 w tumor-bearing mice when treated with 150 μM OA. The relative expression of each gene was calculated using β -actin as the internal reference. F Experimental scheme to detect the influence of inhibiting OA uptake on ascitic B cells. G Comparison of protein expressions of main FA metabolic molecules in 3 w OvCa-bearing mouse ascitic B cells pretreated with BMS and treated with OA. β -Actin was used as the internal control to calculate the relative expression level of the main FA metabolic molecules. H Mean fluorescence intensity of Bodipy C16 in 3 w OvCa-bearing mouse ascitic B cells pretreated with BMS and treated with OA was detected by flow cytometry. I The expression of A-CoA, ATP and the FAO activity in 3 w OvCa-bearing mouse ascitic B cells pretreated with BMS treated with OA was detected by ELISA. J Mean fluorescence intensity of intracellular oxidized lipid in 3 w OvCa-bearing mouse ascitic B cells pretreated with BMS and treated with OA was detected by flow cytometry. K Mean fluorescence intensity of CD80, CD86, and CD83 in 3 w OvCa-bearing mouse ascitic CD19 + B cells pretreated with BMS and treated with OA was detected by flow cytometry. L Mean fluorescence intensity of Bodipy C16, CD80, CD86 and CD83 in FABP4-knockdown CD19 + B cells, which are from the ascites of 3 w OvCa-bearing mice, treated with OA, was detected by flow cytometry. PBMC, peripheral blood mononuclear cell; SP, spleen; AS, Ascites; OA, oleic acid; PA, palmitic acid; BMS, BMS309403. Data are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, not significant

    Journal: Cancer Immunology, Immunotherapy : CII

    Article Title: Promoting APC function of B cells via reprogramming the fatty acid metabolism enhances anticancer immunity in metastatic ovarian cancer

    doi: 10.1007/s00262-026-04387-y

    Figure Lengend Snippet: The APC function and resulting anticancer immunity of B cells can be enhanced by oleic acid (OA) via reprogramming FA metabolism in vitro. A Mean fluorescence intensity of CD80 and MHC Class II molecules in CD19 + B cells from peripheral blood of healthy volunteers (n = 3) treated with OA and PA (both 150 μM), respectively. B Mean fluorescence intensity of CD80, CD86, CD83, MHC Class II molecules, and Ki67 in splenic CD19 + B cells of WT mice treated with 150 μM OA. C Mean fluorescence intensity of CD80, CD86, CD83, and MHC Class II molecules in ascitic CD19 + B cells from 3 w OvCa-bearing mice when treated with 150 μM OA. D Analysis of FA metabolism-related signaling pathways based on RNA-seq results. GSEA was used to analysised the FA metabolic pathways. E Comparison of mRNA levels of main FA metabolic genes in ascitic B cells from 3 w tumor-bearing mice when treated with 150 μM OA. The relative expression of each gene was calculated using β -actin as the internal reference. F Experimental scheme to detect the influence of inhibiting OA uptake on ascitic B cells. G Comparison of protein expressions of main FA metabolic molecules in 3 w OvCa-bearing mouse ascitic B cells pretreated with BMS and treated with OA. β -Actin was used as the internal control to calculate the relative expression level of the main FA metabolic molecules. H Mean fluorescence intensity of Bodipy C16 in 3 w OvCa-bearing mouse ascitic B cells pretreated with BMS and treated with OA was detected by flow cytometry. I The expression of A-CoA, ATP and the FAO activity in 3 w OvCa-bearing mouse ascitic B cells pretreated with BMS treated with OA was detected by ELISA. J Mean fluorescence intensity of intracellular oxidized lipid in 3 w OvCa-bearing mouse ascitic B cells pretreated with BMS and treated with OA was detected by flow cytometry. K Mean fluorescence intensity of CD80, CD86, and CD83 in 3 w OvCa-bearing mouse ascitic CD19 + B cells pretreated with BMS and treated with OA was detected by flow cytometry. L Mean fluorescence intensity of Bodipy C16, CD80, CD86 and CD83 in FABP4-knockdown CD19 + B cells, which are from the ascites of 3 w OvCa-bearing mice, treated with OA, was detected by flow cytometry. PBMC, peripheral blood mononuclear cell; SP, spleen; AS, Ascites; OA, oleic acid; PA, palmitic acid; BMS, BMS309403. Data are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, not significant

    Article Snippet: In the mechanistic study, mouse ascitic CD19 + B cells (1 × 10 6 /ml) were pretreated with fatty acid binding protein 4 gene (FABP4) inhibitor (BMS309403, MedChemExpress; Cat# HY-101903; 50 μM), PPARγ antagonist (GW9662, MedChemExpress; Cat# HY-16578; 25 μM), and PPAR γ agonist (Troglitazone, Trog, MedChemExpress; Cat# HY-50935; 10 μM) for 2h, respectively.

    Techniques: In Vitro, Fluorescence, Protein-Protein interactions, RNA Sequencing, Comparison, Expressing, Control, Flow Cytometry, Activity Assay, Enzyme-linked Immunosorbent Assay, Knockdown

    Reprogramming of the FA metabolism of B cells by OA can improve anticancer immunity in vitro. A Experimental scheme to detect the in vitro effects of ascitic B cells treated with OA on anticancer immunity. B Mean fluorescence intensity of CD80, CD86, and CD83 in 3 w OvCa-bearing mouse ascitic CD19 + B cells after pulsed with ID8-Luc-cell-prepared antigenic peptides and pretreated with BMS and treated with OA, T cells and ID8-Luc. C Levels of IFN- γ , GZMB, and TNF-α in the supernatant of the coculture system constructed by ID8-Luc cells and 3 w OvCa-bearing mouse ascitic B and splenic T cells were detected by ELISA. D Cytotoxicity of T cells in the coculture system mentioned above was detected by luciferase assay. AS, Ascites; Ag, Antigen; OA, oleic acid; BMS, BMS309403. Data are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, not significant

    Journal: Cancer Immunology, Immunotherapy : CII

    Article Title: Promoting APC function of B cells via reprogramming the fatty acid metabolism enhances anticancer immunity in metastatic ovarian cancer

    doi: 10.1007/s00262-026-04387-y

    Figure Lengend Snippet: Reprogramming of the FA metabolism of B cells by OA can improve anticancer immunity in vitro. A Experimental scheme to detect the in vitro effects of ascitic B cells treated with OA on anticancer immunity. B Mean fluorescence intensity of CD80, CD86, and CD83 in 3 w OvCa-bearing mouse ascitic CD19 + B cells after pulsed with ID8-Luc-cell-prepared antigenic peptides and pretreated with BMS and treated with OA, T cells and ID8-Luc. C Levels of IFN- γ , GZMB, and TNF-α in the supernatant of the coculture system constructed by ID8-Luc cells and 3 w OvCa-bearing mouse ascitic B and splenic T cells were detected by ELISA. D Cytotoxicity of T cells in the coculture system mentioned above was detected by luciferase assay. AS, Ascites; Ag, Antigen; OA, oleic acid; BMS, BMS309403. Data are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, not significant

    Article Snippet: In the mechanistic study, mouse ascitic CD19 + B cells (1 × 10 6 /ml) were pretreated with fatty acid binding protein 4 gene (FABP4) inhibitor (BMS309403, MedChemExpress; Cat# HY-101903; 50 μM), PPARγ antagonist (GW9662, MedChemExpress; Cat# HY-16578; 25 μM), and PPAR γ agonist (Troglitazone, Trog, MedChemExpress; Cat# HY-50935; 10 μM) for 2h, respectively.

    Techniques: In Vitro, Fluorescence, Construct, Enzyme-linked Immunosorbent Assay, Luciferase

    The enhanced APC function of B cells by OA in vitro is achieved through H3K27ac-mediated upregulation of PPAR γ expression. A Protein expression of β -actin and H3K27ac in 3 w OvCa-bearing mouse ascitic CD19 + B cells pretreated with BMS and treated with OA was assessed by WB. β -Actin was used as the internal control to calculate the relative expression level of H3K27ac. B The enrichment percentage of H3K27ac at the PPAR γ , CD80, CD86, and CD83 promoter regions was quantified using ChIP-seq analysis. C Correlation analysis of PPAR γ and APC function-related genes (CD80, CD86, CD83, MHC II) in B cells in OvCa patients in the TCGA database (n = 426). D Analysis of protein interaction among FABP4, PPAR γ , CD80, CD86, and CD83. E The enrichment percentage of PPAR γ at the CD80, CD86, and CD83 promoter regions was quantified using ChIP-seq analysis. F Mean fluorescence intensity of CD80, CD86, and CD83 in 3 w OvCa-bearing mouse ascitic CD19 + B cells pretreated with BMS, GW9662/Trog, and treated with OA was detected by flow cytometry. AS, Ascites; Ag, Antigen; OA, oleic acid; BMS, BMS309403. Data are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, not significant

    Journal: Cancer Immunology, Immunotherapy : CII

    Article Title: Promoting APC function of B cells via reprogramming the fatty acid metabolism enhances anticancer immunity in metastatic ovarian cancer

    doi: 10.1007/s00262-026-04387-y

    Figure Lengend Snippet: The enhanced APC function of B cells by OA in vitro is achieved through H3K27ac-mediated upregulation of PPAR γ expression. A Protein expression of β -actin and H3K27ac in 3 w OvCa-bearing mouse ascitic CD19 + B cells pretreated with BMS and treated with OA was assessed by WB. β -Actin was used as the internal control to calculate the relative expression level of H3K27ac. B The enrichment percentage of H3K27ac at the PPAR γ , CD80, CD86, and CD83 promoter regions was quantified using ChIP-seq analysis. C Correlation analysis of PPAR γ and APC function-related genes (CD80, CD86, CD83, MHC II) in B cells in OvCa patients in the TCGA database (n = 426). D Analysis of protein interaction among FABP4, PPAR γ , CD80, CD86, and CD83. E The enrichment percentage of PPAR γ at the CD80, CD86, and CD83 promoter regions was quantified using ChIP-seq analysis. F Mean fluorescence intensity of CD80, CD86, and CD83 in 3 w OvCa-bearing mouse ascitic CD19 + B cells pretreated with BMS, GW9662/Trog, and treated with OA was detected by flow cytometry. AS, Ascites; Ag, Antigen; OA, oleic acid; BMS, BMS309403. Data are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, not significant

    Article Snippet: In the mechanistic study, mouse ascitic CD19 + B cells (1 × 10 6 /ml) were pretreated with fatty acid binding protein 4 gene (FABP4) inhibitor (BMS309403, MedChemExpress; Cat# HY-101903; 50 μM), PPARγ antagonist (GW9662, MedChemExpress; Cat# HY-16578; 25 μM), and PPAR γ agonist (Troglitazone, Trog, MedChemExpress; Cat# HY-50935; 10 μM) for 2h, respectively.

    Techniques: In Vitro, Expressing, Control, ChIP-sequencing, Fluorescence, Flow Cytometry

    The combination of adoptive immunotherapy with APC function-enhanced B cells and LDC improved anticancer immunity in metastatic OvCa mice. A Experimental scheme to evaluate the effects of combining adoptive immunotherapy with APC function-enhanced B cells and LDC in metastatic OvCa mice. B Representative BLI images and comparison of OvCa progression in each group (n = 3). C Representative images ( a ) and the number of tumor nodules ( b ) in the abdominal wall of mice in each group (n = 3). Areas marked by yellow dashed lines: Representative tumor nodules in the abdominal wall. D (a) Representative images of lymphoid aggregates (composed of CD3 + T cells, CD19 + B cells, and CD21 + FDC) in the ascitic cells of mice in each group. (b) Comparison of the amount of CD3 + T, CD8 + T, Ki67 + CD3 + T, Ki67 + CD8 + T, CD19 + B, CD80 + B, CD86 + B, and lymphoid aggregates respectively in the ascitic cells of mice in each group (n = 3) in frozen sections detected by immunofluorescence. E Comparison of the proportion of CD3 + T, CD8 + T, CD19 + B, CD19 − CD138 + B cells, mean fluorescence intensity of Ki67 in CD3 + T and CD8 + T, and mean fluorescence intensity of CD80, CD86, CD83, MHC II in CD19 + B, respectively, in ascitic cells of each group of mice (n = 3) detected by flow cytometry. F Mean fluorescence intensity values of IL-2, IFN- γ , GZMB, and CTLA-4 in CD8 + T cells, respectively. G Kaplan Meier analysis of survival time of each group of mice (n = 7). LDC, low-dose chemotherapy; B OA , B cells that have been treated with OA for 24h; L-DDP, low-dose DDP (1 mg/kg); H-DDP, high-dose DDP (2 mg/kg); BLI, bioluminescence imaging. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, not significant

    Journal: Cancer Immunology, Immunotherapy : CII

    Article Title: Promoting APC function of B cells via reprogramming the fatty acid metabolism enhances anticancer immunity in metastatic ovarian cancer

    doi: 10.1007/s00262-026-04387-y

    Figure Lengend Snippet: The combination of adoptive immunotherapy with APC function-enhanced B cells and LDC improved anticancer immunity in metastatic OvCa mice. A Experimental scheme to evaluate the effects of combining adoptive immunotherapy with APC function-enhanced B cells and LDC in metastatic OvCa mice. B Representative BLI images and comparison of OvCa progression in each group (n = 3). C Representative images ( a ) and the number of tumor nodules ( b ) in the abdominal wall of mice in each group (n = 3). Areas marked by yellow dashed lines: Representative tumor nodules in the abdominal wall. D (a) Representative images of lymphoid aggregates (composed of CD3 + T cells, CD19 + B cells, and CD21 + FDC) in the ascitic cells of mice in each group. (b) Comparison of the amount of CD3 + T, CD8 + T, Ki67 + CD3 + T, Ki67 + CD8 + T, CD19 + B, CD80 + B, CD86 + B, and lymphoid aggregates respectively in the ascitic cells of mice in each group (n = 3) in frozen sections detected by immunofluorescence. E Comparison of the proportion of CD3 + T, CD8 + T, CD19 + B, CD19 − CD138 + B cells, mean fluorescence intensity of Ki67 in CD3 + T and CD8 + T, and mean fluorescence intensity of CD80, CD86, CD83, MHC II in CD19 + B, respectively, in ascitic cells of each group of mice (n = 3) detected by flow cytometry. F Mean fluorescence intensity values of IL-2, IFN- γ , GZMB, and CTLA-4 in CD8 + T cells, respectively. G Kaplan Meier analysis of survival time of each group of mice (n = 7). LDC, low-dose chemotherapy; B OA , B cells that have been treated with OA for 24h; L-DDP, low-dose DDP (1 mg/kg); H-DDP, high-dose DDP (2 mg/kg); BLI, bioluminescence imaging. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, not significant

    Article Snippet: In the mechanistic study, mouse ascitic CD19 + B cells (1 × 10 6 /ml) were pretreated with fatty acid binding protein 4 gene (FABP4) inhibitor (BMS309403, MedChemExpress; Cat# HY-101903; 50 μM), PPARγ antagonist (GW9662, MedChemExpress; Cat# HY-16578; 25 μM), and PPAR γ agonist (Troglitazone, Trog, MedChemExpress; Cat# HY-50935; 10 μM) for 2h, respectively.

    Techniques: Comparison, Immunofluorescence, Fluorescence, Flow Cytometry, Imaging